2V4H
NEMO CC2-LZ domain - 1D5 DARPin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.020, 63.020, 436.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.900 |
| R-factor | 0.21051 |
| Rwork | 0.208 |
| R-free | 0.26821 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jab |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.586 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.130 | 0.450 |
| Number of reflections | 86037 | |
| <I/σ(I)> | 11.6 | 1.8 |
| Completeness [%] | 84.8 | 42.9 |
| Redundancy | 4.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 5% MPD, 5% ETHANOL, 100 MM HEPES, PH 7.5 |
