2V40
Human Adenylosuccinate synthetase isozyme 2 in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 155.410, 155.410, 82.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.370 - 1.900 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1loo |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.200 |
| Number of reflections | 46302 | |
| <I/σ(I)> | 26.9 | 13 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 16.9 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 0.1 M TRIS PH 8.2, 0.2 M TRI-METHYLAMINE N-OXIDE, 24% PEG 2000 MME |
