2V3J
The yeast ribosome synthesis factor Emg1 alpha beta knot fold methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.479, 76.614, 87.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.010 - 2.000 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.935 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD (SOLVE) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.040 | 0.140 |
| Number of reflections | 20207 | |
| <I/σ(I)> | 18.2 | 4 |
| Completeness [%] | 93.2 | 61.1 |
| Redundancy | 3.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25% PEG4K, 0.2M LI2SO4, 0.1M TRIS PH7.5, 20% GLYCEROL |
