2V2V
IspE in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-24 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 137.256, 137.256, 137.256 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.000 - 2.400 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.27200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.191 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.580 |
| Number of reflections | 33908 | |
| <I/σ(I)> | 15.8 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.6 M (NH4)2SO4, 0.1 M NA CACODYLATE, PH 6.5, 0.1 M NABR |
