2V2J
Wild type recombinant horse spleen apoferritin cocrystallized with haemin in basic conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-10 |
| Detector | MARRESEARCH |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.981, 182.981, 182.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.410 - 2.220 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v2i |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.632 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.3.0031) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.300 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.040 | 0.140 |
| Number of reflections | 13290 | |
| <I/σ(I)> | 25.7 | 9.3 |
| Completeness [%] | 98.3 | 97.8 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | RESERVOIR: CADMIUM SULFATE 0.04M, AMMONIUM SULFATE 0.5M, SODIUM AZIDE 0.003M DROP: 1UL PROTEIN AND 1 UL RESERVOIR, pH 8.2 |
