2V2C
The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-28 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 119.230, 59.290, 46.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.010 - 1.890 |
R-factor | 0.142 |
Rwork | 0.139 |
R-free | 0.18200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tim |
RMSD bond length | 0.022 |
RMSD bond angle | 1.714 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0022) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.950 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.030 | 0.060 |
Number of reflections | 27265 | |
<I/σ(I)> | 63.26 | 26.98 |
Completeness [%] | 99.5 | 95.5 |
Redundancy | 8.52 | 6.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M TEA PH 7.5, 2 % PEG 400, 2.0 M (NH4)2SO4 |