2V2C
The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.230, 59.290, 46.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.010 - 1.890 |
| R-factor | 0.142 |
| Rwork | 0.139 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tim |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.714 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0022) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.950 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.030 | 0.060 |
| Number of reflections | 27265 | |
| <I/σ(I)> | 63.26 | 26.98 |
| Completeness [%] | 99.5 | 95.5 |
| Redundancy | 8.52 | 6.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TEA PH 7.5, 2 % PEG 400, 2.0 M (NH4)2SO4 |
