2V1Y
Structure of a phosphoinositide 3-kinase alpha adaptor-binding domain (ABD) in a complex with the iSH2 domain from p85 alpha
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9794,0.9393 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.799, 62.000, 74.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.400 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.29200 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.259 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 2.420 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.200 |
| Number of reflections | 10926 | |
| <I/σ(I)> | 13.48 | 3.32 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 3.53 | 3.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M MG(NO3)2, 20% PEG3350 (HAMPTON), 5MM TRIS-HCL PH 7.0 (25C), 5% GLYCEROL AND 5MM FRESH DTT |
