2V1X
Crystal structure of human RECQ-like DNA helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.229, 97.647, 85.849 |
| Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.000 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.740 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 89784 | |
| <I/σ(I)> | 10.4 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M SODIUM BROMIDE,20% PEG3350, 10% ETHYLENE GLYCOL, 0.1M BIS TRIS PROPANE PH7.5, pH 7.50 |
