Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-11 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 228.942, 66.363, 146.419 |
Unit cell angles | 90.00, 102.80, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.22 |
Rwork | 0.218 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w0e |
RMSD bond length | 0.080 |
RMSD bond angle | 1.175 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.380 |
Number of reflections | 52714 | |
<I/σ(I)> | 7.6 | 5.5 |
Completeness [%] | 98.8 | 98.1 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | HANGING DROP CRYSTALLISATION USING 1:1 RATIO OF PROTEIN AT 20-27 MG/ML IN A SOLUTION CONTAINING 50 MM POTASSIUM PHOSPHATE PH 7.2, 0.2 MM POTASSIUM CHLORIDE, 20% (V/V) GLYCEROL, 2 MM DITHIOTHREITOL, 1MM EDTA |