2V08
Structure of wild-type Phormidium laminosum cytochrome c6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 57.364, 57.364, 89.553 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.590 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.184 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctj |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.779 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.500 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.230 |
| Number of reflections | 11288 | |
| <I/σ(I)> | 12.3 | 4.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2.5 M NACL, 100 MM IMIDAZOLE, 200 MM ZN(OAC)2, PH 6.0. |
