2V06
Crystal structure of the PPM Ser-Thr phosphatase MsPP from Mycobacterium smegmatis at pH 5.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-03 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.718, 83.596, 33.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.050 |
R-factor | 0.1314 |
R-free | 0.18750 |
Structure solution method | OTHER |
RMSD bond length | 0.014 |
RMSD bond angle | 0.035 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.100 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.030 | 0.410 |
Number of reflections | 97914 | |
<I/σ(I)> | 11.3 | 2.7 |
Completeness [%] | 98.8 | 99.2 |
Redundancy | 2.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | pH 5.5 |