2V03
High resolution structure and catalysis of an O-acetylserine sulfhydrylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 76.615, 76.615, 209.787 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.330 |
R-factor | 0.158 |
Rwork | 0.158 |
R-free | 0.17200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.711 |
Refinement software | REFMAC (5.3.0022) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 63.000 |
High resolution limit [Å] | 1.330 |
Rmerge | 0.060 |
Number of reflections | 83156 |
Completeness [%] | 98.6 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.2 | pH 5.2 |