2UZZ
X-ray structure of N-methyl-L-tryptophan oxidase (MTOX)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 88.188, 89.809, 91.915 |
| Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| R-factor | 0.24 |
| Rwork | 0.236 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1el5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.194 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.220 |
| Number of reflections | 21160 | |
| <I/σ(I)> | 7 | 4 |
| Completeness [%] | 92.0 | 93.8 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | PEG4000 20%, ISOPROPANOL 20%, SODIUM CITRATE 0.1 M, PH=5.6 |
