2UYV

L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT Q6Y- E192A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-15
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	95.565, 102.355, 265.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.2043
Rwork	0.204
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ojr
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.380
Number of reflections	62025
<I/σ(I)>	15.15	3.87
Completeness [%]	98.6	99.7
Redundancy	4.12	4.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.7		1.0 M NA/K-TARTRATE, 0.1 M HEPES (PH 7.7)