2UYG
Crystallogaphic structure of the typeII 3-Dehydroquinase from Thermus Thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-19 |
| Detector | JUPITER 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.085, 151.586, 159.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.200 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gqo |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.534 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.290 |
| Number of reflections | 103688 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.3 | 94.4 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.8M AMM3CITRATE,0.1M BIS-TRIS PEOPANE(PH7) |
