2UWE
Large CDR3a loop alteration as a function of MHC mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.489, 84.178, 121.773 |
| Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
| Resolution | 121.000 - 2.400 |
| R-factor | 0.243 |
| Rwork | 0.240 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lp9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.040 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.370 |
| Rmerge | 0.060 | 0.350 |
| Number of reflections | 76682 | |
| <I/σ(I)> | 8.3 | 1.64 |
| Completeness [%] | 83.9 | 67.2 |
| Redundancy | 2.76 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 14% PEG 8000, 1 M NACL, 25 MM HEPES, PH 7.8 |
