Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 87 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.000, 78.700, 128.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.186 * |
| Rwork | 0.186 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 15.000 * |
| Data scaling software | XSCALIBRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 1.800 | 1.800 * |
| Rmerge | 0.046 | 0.230 * |
| Total number of observations | 146373 * | |
| Number of reflections | 68170 | 7563 * |
| Completeness [%] | 97.0 | 87 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 20 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 5 (mM) | |
| 3 | 1 | drop | UDP-phenol | 2.5 (mM) | |
| 4 | 1 | drop | PEG8000 | 10 (%) | |
| 5 | 1 | drop | HEPES | 25 (mM) | |
| 6 | 1 | drop | 250 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 20 (%) | |
| 8 | 1 | reservoir | HEPES | 50 (mM) | |
| 9 | 1 | reservoir | 500 (mM) |
