2TCL

STRUCTURE OF THE CATALYTIC DOMAIN OF HUMAN FIBROBLAST COLLAGENASE COMPLEXED WITH AN INHIBITOR

Experimental procedure

Spacegroup name	I 4
Unit cell lengths	86.960, 86.960, 40.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.200
R-factor	0.21 *
Rwork	0.162
RMSD bond length	0.013 *
RMSD bond angle	3.000 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.065 *
Total number of observations	18085 *
Number of reflections	7339 *
Completeness [%]	92.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		2 (mM)
4	1	reservoir	PEG4000	25 (%)
5	1	reservoir	sodium cacodylate	0.1 (M)
6	1	reservoir	ammonium acetate	0.2 (M)