2SRC
CRYSTAL STRUCTURE OF HUMAN TYROSINE-PROTEIN KINASE C-SRC, IN COMPLEX WITH AMP-PNP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.590, 72.970, 172.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 * |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fmk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.270 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.051 * |
| Total number of observations | 296913 * |
| Number of reflections | 55186 |
| Completeness [%] | 93.2 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 11 | 1 | reservoir | PEG4000 | 8 (%) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | AMP-PNP | 0.1 (M) | |
| 6 | 1 | drop | peptide | 22 (mM) | |
| 7 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 8 | 1 | reservoir | MES | 50 (mM) | |
| 9 | 1 | reservoir | 10 (mM) |
