2RKA

The Structure of rat cytosolic PEPCK in complex with phosphoglycolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	118
Detector technology	IMAGE PLATE
Collection date	2007-05-29
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 1 21 1
Unit cell lengths	60.670, 119.660, 90.867
Unit cell angles	90.00, 108.90, 90.00

Refinement procedure

Resolution	29.920 - 1.950
R-factor	0.185
Rwork	0.183
R-free	0.22200
RMSD bond length	0.008
RMSD bond angle	1.125
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.105	0.046	0.705
Number of reflections	86353
<I/σ(I)>	9.5
Completeness [%]	96.5	99.5	94.6
Redundancy	6.8	7.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	12-30% PEG 3350, 0.1M HEPES, 10 MM MNCL2, pH 7.4, temperature 298K, VAPOR DIFFUSION, HANGING DROP