2RJF
Crystal structure of L3MBTL1 in complex with H4K20Me2 (residues 12-30), orthorhombic form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-10 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.458, 117.809, 132.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.650 - 2.050 |
| R-factor | 0.18142 |
| Rwork | 0.179 |
| R-free | 0.22533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pqw |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.727 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.650 |
| High resolution limit [Å] | 2.050 |
| Rmerge | 0.082 |
| Number of reflections | 86421 |
| Completeness [%] | 97.4 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | 5% PEG 4000, 0.1M Sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
