2RJ3
Crystal Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with Uracil and Phosphate Ion at 2.49A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-25 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.000, 124.260, 133.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.980 - 2.510 |
Rwork | 0.221 |
R-free | 0.25652 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oec |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.840 |
High resolution limit [Å] | 2.490 |
Number of reflections | 47321 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | VAPOR DIFFUSION, HANGING DROP, temperature 297K |