2RFJ
Crystal structure of the bromo domain 1 in human bromodomain containing protein, testis specific (BRDT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97866 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.935, 92.679, 100.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.063 - 2.050 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nxb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.517 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.063 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.129 | 0.681 |
| Total number of observations | 8196 | |
| Number of reflections | 24939 | |
| <I/σ(I)> | 4.6 | 1 |
| Completeness [%] | 96.4 | 77.6 |
| Redundancy | 6.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.20M Na/K tartrate, 20.0% PEG 3350, 10.0% Ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
