2RFB
Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.7400, 1.6475 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.790, 168.460, 88.230 |
| Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
| Resolution | 48.510 - 2.500 |
| R-factor | 0.21434 |
| Rwork | 0.211 |
| R-free | 0.26844 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.510 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.051 | 0.661 |
| Number of reflections | 48305 | |
| <I/σ(I)> | 31.1 | 2 |
| Completeness [%] | 98.1 | 95.1 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2M ammonium sulfate, 0.1M HEPES, 2% polyethylene glycol 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
