2RC3
Crystal structure of CBS domain, NE2398
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.91970 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.195, 95.641, 99.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.18807 |
| Rwork | 0.188 |
| R-free | 0.23595 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.137 | 0.682 |
| Number of reflections | 67993 | |
| <I/σ(I)> | 7.8 | 1.57 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 11.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 297 | 0.1M Na(OAC) pH 5.2, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
