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2RB4

Crystal structure of the Helicase domain of human DDX25 RNA helicase

Replaces:  2G2J
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBESSY BEAMLINE 14.2
Synchrotron siteBESSY
Beamline14.2
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2006-01-16
DetectorMACSCIENCE
Wavelength(s)0.95373
Spacegroup nameP 43 21 2
Unit cell lengths70.310, 70.310, 187.120
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.440 - 2.800
R-factor0.23607
Rwork0.233
R-free0.26655
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1fuk
RMSD bond length0.010
RMSD bond angle1.244
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.900
High resolution limit [Å]2.8002.800
Rmerge0.1070.820
Number of reflections12185
<I/σ(I)>15.652.59
Completeness [%]100
Redundancy8.89.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.52930.1 M Bis-Tris propane, 2.5 M Ammonium sulfate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

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