2R8O

Transketolase from E. coli in complex with substrate D-xylulose-5-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.8423
Spacegroup name	P 21 21 21
Unit cell lengths	90.138, 101.951, 133.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.140 - 1.470
R-factor	0.15
Rwork	0.150
R-free	0.16400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.280
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.400	99.000	1.490
High resolution limit [Å]	1.470	4.570	1.470
Rmerge	0.067	0.035	0.317
Number of reflections	207914
<I/σ(I)>	11.6
Completeness [%]	99.8	99.3	99.2
Redundancy	11.3	11.5	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	281	PEG 6000, glycerol, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 281K