2R8F
Crystal structure of H225A NSP2 and ATP-gS complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9790903 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 108.644, 108.644, 155.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Rwork | 0.216 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.240 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.281 |
| Number of reflections | 11744 | |
| <I/σ(I)> | 14.9 | 5.1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 7.81 | 7.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 6-8% PEG 10000, 20mM Tris-HCl pH 7.5, 10-15mM nucleotide, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
