2R7P

Crystal Structure of H225A NSP2 and AMPPNP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790903
Spacegroup name	I 4 2 2
Unit cell lengths	108.430, 108.430, 154.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
Rwork	0.223
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l9v
RMSD bond length	0.007
RMSD bond angle	1.250
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.115	0.395
Number of reflections	11643
<I/σ(I)>		5
Completeness [%]	99.5	100
Redundancy	13.9	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	6-8% PEG 10000, 20mM Tris-HCl pH 7.5, 10-15mM nucleotide, VAPOR DIFFUSION, HANGING DROP, temperature 298K