2R7C
Crystallographic and biochemical analysis of rotavirus NSP2 with nucleotides reveals an NDP kinase like activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793990 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 108.175, 108.175, 151.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.060 - 2.700 |
| Rwork | 0.226 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.336 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.057 | 0.393 |
| Number of reflections | 12530 | |
| <I/σ(I)> | 18.6 | 14.3 |
| Completeness [%] | 98.8 | |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 6-8% PEG 10000, 20mM Tris-HCl pH 7.5, 10-15mM nucleotide, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
