2R4V
Structure of human CLIC2, crystal form A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.015, 74.722, 79.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.550 - 1.850 |
R-factor | 0.21665 |
Rwork | 0.215 |
R-free | 0.25038 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k0m |
RMSD bond length | 0.016 |
RMSD bond angle | 1.538 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.550 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.060 | 0.300 |
Number of reflections | 23144 | |
<I/σ(I)> | 31.3 | 2.8 |
Completeness [%] | 93.9 | 62 |
Redundancy | 10.5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 35%-50% PEG 400, 100mM Tris-HCl, pH8.0, 5mM GSH, VAPOR DIFFUSION, temperature 293K |