2R4R
Crystal structure of the human beta2 adrenoceptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 338.380, 48.480, 89.350 |
Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
Resolution | 19.990 - 3.400 |
Rwork | 0.217 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gzm and 1igt |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.400 | 3.490 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.117 | 0.407 |
Number of reflections | 19671 | |
<I/σ(I)> | 9.9 | 2.3 |
Completeness [%] | 98.9 | 94.9 |
Redundancy | 3.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |