2R4H
Crystal structure of a C1190S mutant of the 6th PDZ domain of human membrane associated guanylate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.006029 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.052, 77.759, 50.182 |
| Unit cell angles | 90.00, 101.49, 90.00 |
Refinement procedure
| Resolution | 38.870 - 2.050 |
| R-factor | 0.19148 |
| Rwork | 0.188 |
| R-free | 0.25829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jik |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.683 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.250 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.133 | 0.683 |
| Number of reflections | 17538 | |
| <I/σ(I)> | 9.1 | 2.1 |
| Completeness [%] | 92.6 | 99.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20% PEG 3350, 0.1M Citrate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
