2R34
Crystal structure of MN human arg-insulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 80.687, 80.687, 37.517 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.360 - 2.250 |
R-factor | 0.193 |
Rwork | 0.186 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1trz |
RMSD bond length | 0.037 |
RMSD bond angle | 2.611 |
Data reduction software | AUTOMAR |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.150 | 0.590 |
Number of reflections | 4331 | |
<I/σ(I)> | 3.3 | 1 |
Completeness [%] | 96.3 | 95.3 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | Sodium Citrate, Ammonium Sulphate, Magnesium Chloride, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |