2R32
Crystal Structure of human GITRL variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.072 |
| Spacegroup name | H 3 |
| Unit cell lengths | 61.219, 61.219, 113.948 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.060 - 1.950 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24600 |
| Starting model (for MR) | 2q1m |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.534 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.077 | 0.074 | 0.491 |
| Number of reflections | 11656 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 99.8 | 98.9 | 99.6 |
| Redundancy | 5.9 | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2.0 M Ammonium Sulphate, Vapor diffusion, Sitting drop, temperature 298K |
