2R2W

Urokinase plasminogen activator B-chain-GPPE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-09-22
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	53.310, 54.760, 82.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.174 - 2.010
Rwork	0.199
R-free	0.23300
RMSD bond length	0.006
RMSD bond angle	1.249
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.174	24.500	2.120
High resolution limit [Å]	2.010	6.360	2.010
Rmerge	0.088	0.063	0.316
Total number of observations		1315	5277
Number of reflections	16087
<I/σ(I)>	5.8	6.6	2.3
Completeness [%]	97.0	90.7	90.7
Redundancy	2.5	2.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	277	sodium citrate, ammonium sulfate, lithium sulfate, pH 5.2, vapor diffusion, hanging drop, temperature 277K