2R2U
Co(III)bleomycinB2 bithiazole/C-terminal tail domain bound to d(ATTTAGTTAACTAAAT) complexed with MMLV RT catalytic fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2007-02-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.068828 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 55.689, 146.191, 46.931 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
Rwork | 0.241 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ztw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.246 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.072 | 0.039 | 0.339 |
Number of reflections | 16721 | ||
<I/σ(I)> | 11.1 | ||
Completeness [%] | 96.1 | 95.7 | 81.3 |
Redundancy | 4.2 | 4.4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | PEG 4000, MgCl2, pH 6.5, vapor diffusion, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 2 | PEG 4000 | ||
4 | 1 | 2 | MgCl2 |