2R2I
Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.5498 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.333, 73.333, 73.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.930 - 2.000 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.25100 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 13602 | |
Completeness [%] | 96.7 | 98.2 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 24% PEG 20,000, 0.4 M potassium nitrate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 24% PEG 20,000, 0.4 M potassium nitrate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |