2R26
The Structure of the Ternary Complex of Carboxymethyl Coenzyme A and Oxalateacetate with Citrate Synthase from the Thermophilic Archaeonthermoplasma Acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.705, 74.221, 89.874 |
| Unit cell angles | 99.34, 98.34, 114.38 |
Refinement procedure
| Resolution | 43.090 - 2.500 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26100 |
| Starting model (for MR) | 1O7X_A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.981 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.090 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.101 |
| Number of reflections | 58219 |
| <I/σ(I)> | 6.1 |
| Completeness [%] | 97.5 |
| Redundancy | 3.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 280 | 15-18% Peg 400, 100mM Hepes 7-8.5, 200 mM Sodium Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
