2R24
Human Aldose Reductase structure
Experimental procedure
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 298 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.066, 67.126, 47.862 |
Unit cell angles | 90.00, 92.41, 90.00 |
Refinement procedure
Resolution | 40.110 - 2.194 |
R-factor | 0.2596 |
Rwork | 0.257 |
R-free | 0.29120 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.759 |
Data reduction software | LAUEGEN |
Data scaling software | LSCALE |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.110 |
High resolution limit [Å] | 1.750 |
Number of reflections | 46319 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 297 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 297 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |