2R0V
Structure of the Rsc4 tandem bromodomain acetylated at K25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.246, 83.169, 127.059 |
| Unit cell angles | 90.00, 109.27, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.283 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.076 | 0.503 |
| Number of reflections | 50527 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 298 | 0.1M Tris, 0.2M ammonium sulfate, 22% PEG 4000, pH 8.0, VAPOR DIFFUSION, temperature 298K, pH 8.00 |
