2R00
crystal structure of aspartate semialdehyde dehydrogenase II complexed with ASA from vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.376, 84.679, 114.976 |
| Unit cell angles | 90.00, 102.07, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.030 |
| R-factor | 0.19319 |
| Rwork | 0.191 |
| R-free | 0.23237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ys4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.187 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 70525 | |
| <I/σ(I)> | 27 | 5 |
| Completeness [%] | 99.7 | 96.1 |
| Redundancy | 3.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M sodium citrate pH 5.5, 27% PEG8000, 0.3M ammonium acetate, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
