2QZV
Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-01-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 631.449, 464.724, 584.572 |
| Unit cell angles | 90.00, 123.84, 90.00 |
Refinement procedure
| Resolution | 200.000 - 9.000 |
| Rwork | 0.615 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | CRYO EM DENSITY |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | DM |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 9.500 |
| High resolution limit [Å] | 9.000 | 9.000 |
| Number of reflections | 101681 | |
| <I/σ(I)> | 6.5 | 1.2 |
| Completeness [%] | 98.1 | 95.5 |
| Redundancy | 6.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.5% PEG 8000, 3% GLYCEROL, 0.05M NA MOPS, 0.04M MGCL2, 0.2% N-OCTYL-BETA-D-GLUCOPYRANOSIDE, INITIALLY SATURATED WITH CYCLOHEXANE, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, PH 6.80 |
