2QUX
PP7 coat protein dimer in complex with RNA hairpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9797 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.970, 145.388, 109.655 |
| Unit cell angles | 90.00, 122.94, 90.00 |
Refinement procedure
| Resolution | 33.800 - 2.440 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qud |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.596 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 2.540 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.065 | 0.450 |
| Number of reflections | 85663 | |
| <I/σ(I)> | 18.7 | 2.3 |
| Completeness [%] | 98.6 | 87.6 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 15% PEG 3350, 0.1M Na Citrate, 0.01M MES, 0.001M Cobaltous chloride hexahydrate, 0.18M Ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 10 | 1 | 2 | Ammonium sulfate | ||
| 2 | 1 | 1 | Na Citrate | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | Cobaltous chloride hexahydrate | ||
| 5 | 1 | 1 | Ammonium sulfate | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | Na Citrate | ||
| 8 | 1 | 2 | MES | ||
| 9 | 1 | 2 | Cobaltous chloride hexahydrate |
