2QUK
Crystal structures of human tryptophanyl-tRNA synthetase in complex with ATP(putative)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 77 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 235.600, 235.600, 48.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.267 |
Rwork | 0.267 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o5t |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.930 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.088 | 0.405 |
Number of reflections | 20030 | |
<I/σ(I)> | 21.1 | 2.6 |
Completeness [%] | 99.1 | 93.9 |
Redundancy | 7.6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | PEG3350, PEG1500, Na Citrate,, pH8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |