2QUJ

Crystal structures of human tryptophanyl-tRNA synthetase in complex with TrpAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	77
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 4 2 2
Unit cell lengths	80.000, 80.000, 383.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.420
R-factor	0.214
Rwork	0.214
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o5t
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.068	0.289
Number of reflections	43231
<I/σ(I)>	25	2.9
Completeness [%]	88.1	43
Redundancy	6.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	1.0M Na Citrate, 100mM HEPES, pH7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K