2QUF
Crystal Structure of Transcription Factor AXXA-PF0095 from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.747, 93.484, 71.062 |
Unit cell angles | 90.00, 102.44, 90.00 |
Refinement procedure
Resolution | 32.530 - 2.800 |
R-factor | 0.246 |
Rwork | 0.244 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2plz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.058 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.530 | 2.880 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 11712 | |
Completeness [%] | 98.0 | 85 |
Redundancy | 6.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.8 | 291 | 70mM Sodium Acetate, 30% Glycerol, pH 4.8, modified microbatch, temperature 291K |