2QTS
Structure of an acid-sensing ion channel 1 at 1.9 A resolution and low pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 124.252, 110.781, 149.891 |
| Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.2081 |
| Rwork | 0.208 |
| R-free | 0.23300 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.420 |
| Number of reflections | 295827 | |
| <I/σ(I)> | 13.6 | 1.8 |
| Completeness [%] | 93.6 | 69.3 |
| Redundancy | 3.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1M ADA (pH 6.5), 0.1M MgCl2, 12% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
