2QTS
Structure of an acid-sensing ion channel 1 at 1.9 A resolution and low pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 124.252, 110.781, 149.891 |
Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.2081 |
Rwork | 0.208 |
R-free | 0.23300 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.055 | 0.420 |
Number of reflections | 295827 | |
<I/σ(I)> | 13.6 | 1.8 |
Completeness [%] | 93.6 | 69.3 |
Redundancy | 3.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1M ADA (pH 6.5), 0.1M MgCl2, 12% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |