2QRA
Crystal structure of XIAP BIR1 domain (P21 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2002-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.563, 72.983, 68.904 |
| Unit cell angles | 90.00, 95.69, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| Rwork | 0.196 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2poi |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.760 | 2.500 |
| Rmerge | 0.041 | 0.031 | 0.097 |
| Number of reflections | 12816 | ||
| <I/σ(I)> | 33.5 | ||
| Completeness [%] | 97.9 | 95.2 | 75.3 |
| Redundancy | 3.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 15% ethanol, 100mM sodium citrate, 200mM Li2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
