2QPF
Crystal Structure of Mouse Transthyretin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.511, 120.511, 113.186 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.880 - 2.050 |
| R-factor | 0.20043 |
| Rwork | 0.198 |
| R-free | 0.24609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb code 1dvq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.076 | 0.897 |
| Number of reflections | 60024 | |
| <I/σ(I)> | 32.5 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 25% PEG 2000 MME, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
